- Overview of lambda library construction
- Digest of genomic DNA for library construction
- Phage preps via gradient centrifugation
- Phage preps from plates
- Phage preps via liquid lysis
- Phage miniprep
- Amplification of lambda library
- Making packaging extracts
- In vivo excision of lambda ZAP
- Plating and titering lambda phage
- Screening a lambda library
Langdale Lab 