- Acetone precipitation of proteins
- Affinity purification of antibodies using His-tagged protein
- Affinity purification of antibodies using CNBr-sepharose
- Staph A purification of antibodies
- Strip membrane purification of antibodies
- Immobilization of proteins on CNBr-Sepharose
- Optimizing expression of recombinant proteins (E. coli)
- Purification of GST fusion proteins (E. coli)
- Purification of proteins using intein (IMPACT system; E. coli)
- Determining localization of fusion protein (E. coli)
- Purification of insoluble His-tagged proteins
- Purification of soluble His-tagged proteins
- Protein isolation from plant tissue
- Protein preparation for 2D gels
- Polyacrylamide gels
- Coomassie staining protein gels
- Silver staining protein gels
- Western blotting
- Immunoreaction for western blots
- Staining proteins on membranes
Langdale Lab 